An extra time penalty from Toronto FC Academy's Martin Davis built on a first half Alexander Zis strike to give the Junior Reds a tight 2-1 victory over ANB.
"The key moment was having a questionable penalty call in the last minute of the game and TFC converting to obtain full points at the final whistle," said ANB assistant coach, Marcelo Almeida.
"Both teams played hard and a tie would have been a fair result. Always difficult to walk away with nothing with the last kick of the game."
ANB's lone marker was counted as an own goal after striker Jason Mills centred a ball that deflected into the net off a TFCA defender.
"TFC is a strong, disciplined opponent that has been able to obtain results in the past few weeks," added Almeida. "We knew the game would be difficult and the ANB players performed strongly, again displaying good quality passing movements throughout the game."
The defending 2014 League1 Ontario champions, Toronto FC Academy will now host Oakville Blue Devils on Sunday September 27, 1:45 p.m. kickoff, in a match that could hand the 2015 title to the Devils - if the visitors win.
ANB Futbol will play Master's FA Saints on Saturday September 26 at the Ontario Soccer Centre, Vaughan - 9 p.m. kickoff.